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Vedolizumab Drug Level ELISA

$1,620.00
Catalog
30-VELHU-E01
The Vedolizumab Drug Level ELISA is intended for the quantitative determination of free Entyvio® concentration in human serum and EDTA plasma. Research Use Only. Not for Use in Diagnostic Procedures.
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Vedolizumab Drug Level ELISA Instructions for Use
Vedolizumab Drug Level ELISA Instructions for Use
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Vedolizumab Drug Level ELISA Safety Data Sheet
Vedolizumab Drug Level ELISA Safety Data Sheet
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Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
15-1000 ng/mL
Sensitivity
15 ng/mL
Sizes
96 Wells
Sample Types
EDTA Plasma, Serum
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
100
Detection
Colorimetric
This ELISA is designed to quantitate free vedolizumab (therapeutic antibody against α4β7 integrin, e. g. Entyvio®) in EDTA plasma or serum samples. In a first incubation step, the free vedolizumab from the sample is bound to the specific monoclonal anti-vedolizumab antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, Peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free vedolizumab in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standard. The concentrations of free vedolizumab in the samples are determined directly from this curve.

Vedolizumab Drug Level ELISA

30-VELHU-E01

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